Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Methods for Detecting Circulating Cancer Stem Cells (CCSCs) as a Novel Approach for Diagnosis of Colon Cancer Relapse/Metastasis

doi: 10.1038/labinvest.2014.133

Figure Lengend Snippet: A) Diagnostic scheme for isolating nucleated epithelial cells from the blood of mice. Three samples of blood/mouse group were collected and analyzed by FACSorting for percent cells positive or negative for CD45 in the supernatant/buffy coat and red blood cell layers. CD45− cells isolated from the supernatant/buffy coat layer are stained by IF staining for the indicated markers. B) Representative data from FACSorting of the cells into CD45+/− populations, showing forward scatter plots for the two fractions of mouse plasma from GroupIII mice. C) Bar graphs depicting percent cells positive for the specific markers/mL blood, collected from all 3 groups. Images of CD45− cells stained by IF for DCLK1/ANXA2/CD44/LGR5 markers are shown above the bar graphs and co-expression is depicted as merged images on the right. Mean±SEM of data obtained from ~10 slides/3 samples/group. *=P< 0.05 versus normal values.

Article Snippet: Three kits from Stem Cell Technologies were used: 1) RosetteSepTM Human CD45 Depletion Cocktail (#1522), 2) RosetteSepTM Human Circulating Epithelial Tumor Cell Enrichment Cocktail (#15127), and 3) EasySepTM Human Whole Blood CD45 Depletion Kit (#18289), (Vancouver, Canada).

Techniques: Diagnostic Assay, Isolation, Staining, Clinical Proteomics, Expressing

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Methods for Detecting Circulating Cancer Stem Cells (CCSCs) as a Novel Approach for Diagnosis of Colon Cancer Relapse/Metastasis

doi: 10.1038/labinvest.2014.133

Figure Lengend Snippet: Bar graphs illustrating: A) Number of recovered CD45+ cells/mL in the blood of patients, who were either free of colonic growths (normal), or positive for Adenocarcinomas (AdCA); IF images of remaining CD45+ cells collected in the final elution are shown above the bar graphs. B) Number of CD44+ cells/mL in blood of normal vs AdCA patients. C) Number of cells co-localizing for CD45/CD44 per mL of blood. Representative IF staining of circulating cells stained with indicated markers are shown above bar graphs, after negative selection of the samples. D) Number of CD44+ cells/mL, which do not co-localize with CD45 in blood of normal vs AdCA patients; representative IF images are shown above bar graphs. Each bar graph=mean ±SEM of data from 5–7 patients analyzed in triplicate as described in Methods. *=P< 0.05 versus normal values.

Article Snippet: Three kits from Stem Cell Technologies were used: 1) RosetteSepTM Human CD45 Depletion Cocktail (#1522), 2) RosetteSepTM Human Circulating Epithelial Tumor Cell Enrichment Cocktail (#15127), and 3) EasySepTM Human Whole Blood CD45 Depletion Kit (#18289), (Vancouver, Canada).

Techniques: Staining, Selection

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Methods for Detecting Circulating Cancer Stem Cells (CCSCs) as a Novel Approach for Diagnosis of Colon Cancer Relapse/Metastasis

doi: 10.1038/labinvest.2014.133

Figure Lengend Snippet: A) Blood from normal and AdCA patients were processed with the EasySep™ kit, catalog #18289 Cells collected after final elution, were cytospun onto glass slides, followed by IF staining for indicated markers. Based on the staining results, number of cells positive for the indicated markers was determined/mL blood, as shown in the bar graphs. B) Representative IF images showing co-expression of DCLK1/CD45, CD44/CD45 and ANXA2/CD45. Images were taken at 10x magnification and a single cell image was magnified and is presented in an inset. (n=7 normal samples and n=7 AdCA samples).

Article Snippet: Three kits from Stem Cell Technologies were used: 1) RosetteSepTM Human CD45 Depletion Cocktail (#1522), 2) RosetteSepTM Human Circulating Epithelial Tumor Cell Enrichment Cocktail (#15127), and 3) EasySepTM Human Whole Blood CD45 Depletion Kit (#18289), (Vancouver, Canada).

Techniques: Staining, Expressing

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Methods for Detecting Circulating Cancer Stem Cells (CCSCs) as a Novel Approach for Diagnosis of Colon Cancer Relapse/Metastasis

doi: 10.1038/labinvest.2014.133

Figure Lengend Snippet: A) Representative IF images of circulating cancer stem cells, positive for indicated markers. Images were digitally enhanced, as shown by dotted arrows. B) Bar graphs illustrating number of cells/mL, positive for indicated markers, excluding CD45+ cells. (n=7 normal samples and n=7 AdCA samples).

Article Snippet: Three kits from Stem Cell Technologies were used: 1) RosetteSepTM Human CD45 Depletion Cocktail (#1522), 2) RosetteSepTM Human Circulating Epithelial Tumor Cell Enrichment Cocktail (#15127), and 3) EasySepTM Human Whole Blood CD45 Depletion Kit (#18289), (Vancouver, Canada).

Techniques: